Table 3.

De novo fibril formation at 72 h assessed by BCA assay

Shown are the fibril concentrations (μg/ml) obtained when 2000 μg/ml WT and mutant C2-α-syn monomer preparations were incubated at 37 °C with shaking for 72 h, which produces fibril formation by spontaneous nucleation (mean ± S.D.). Differences in the amount of fibrils accumulating in the de novo fibril formation system are similar to the differences observed for WT and mutant fibril accumulation in the homologous SeGMA assays.

WT	A30P	A53T	E46K	G51D	H50Q
912 ± 93 (n = 9)	543 ± 171 (n = 4)	1304 ± 224 (n = 5)	604 ± 286 (n = 9)	423 ± 159 (n = 5)	1320 ± 76 (n = 8)