Table 4.

FlAsH fluorescence intensity in the presence of equal concentrations of WT and mutant C2-α-syn fibrils as a percentage of WT value

To examine whether differences in FlAsH sensitivity for WT and mutant fibril species contribute to differences in measured rates of fibril growth, fluorescence was measured when equal concentrations of WT and mutant fibrils (14.5 μg/ml) were incubated with FlAsH dye. Data were normalized to fluorescence obtained from WT C2-α-syn fibrils. Similarities in fluorescence signal between WT and mutant fibril species support the use of FlAsH for reliable comparisons of WT and mutant fibril growth rates (mean ± S.D., n = 6, independent experiments = 2).

WT	A30P	A53T	E46K	G51D	H50Q
%	%	%	%	%	%
100 ± 15	85.2 ± 16	82.7 ± 29	107.6 ± 6.9	72 ± 5.6	106.9 ± 15.4