Table 5.
Rates of homologous and heterologous fibril growth in 3-h SeGMA assays
Rates of seed growth for homologous and heterologous seed plus monomer combinations were measured by FlAsH fluorescence for three different seed concentrations (2.8–11.5 μg/ml) and a constant monomer concentration of 500 μg/ml. Average rates were expressed as a percentage of the rate obtained for WT seeds extended by WT monomer.
| Rate of seed extension (%WT) at 3 h, mean (95% CI) |
||||||
|---|---|---|---|---|---|---|
| WT | A30P | A53T | E46K | G51D | H50Q | |
| % | % | % | % | % | % | |
| Homologous: matched seeds + monomer | 100 (91.7–108.3) | 23 (18.6–27.4) | 552.6 (481.6–623.6) | 48 (42.5–53.5) | 21 (17.8–24.2) | 323 (302–344) |
| Heterologous: WT seeds + mutant monomer | 4.7 (0–9.6) | 3.7 (0–8.7) | 0.3 (0–1.1) | 2.9 (1.7–4.1) | 97 (91.4–102.6) | |
| Heterologous: mutant seeds + WT monomer | 30 (26.4–33.6) | 17.7 (6.2–29.2) | 16 (13–19) | 22 (19–25) | 7.6 (2.4–12.8) | |