Figure 4.

Effect of ubiquitin-proteasome system inhibitors on SEC fractionation of substrate-bound Hrd1 complexes. A, LiCOR quantification of immunoblot band intensities from SEC fractions, normalized to the peak fraction, and plotted as the percentage of the total. B, CD147 immunoblot analysis of SEC fractionated Hrd1 complexes isolated from DDM solubilized lysates of Hrd1.KI cells left untreated or treated for 2 h with either 10 μm MG132, 5 μm CB-5083, or 10 μm C1. CG, core-glycosylated. The arrowhead between 12 and 12.5 ml indicates where images of two separate immunoblots prepared in parallel were digitally spliced together.