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Analysis of Hrd1 complex stoichiometry by AQUA-MS to determine the amount of Hrd1 relative to interactor
Stoichiometry of Hrd1 relative to the indicated proteins within the indicated SEC fractions is shown.
| Interactor | SEC elution volume |
|||||||
|---|---|---|---|---|---|---|---|---|
| 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | |
| ml | ||||||||
| Fam8A1 | 2.0 | 1.6 | 2.0 | 3.4 | 8.1 | 4.4 | 1.1 | 0.6 |
| SEL1L | 24.3 | 33.1 | 20.0 | 9.9 | 8.6 | 5.2 | 50.9 | 54.3 |
| OS9 | 6.6 | 11.6 | 9.5 | 4.5 | 3.6 | 3.6 | 33.7 | 16.9 |
