Figure 8.

Enzyme reaction products from ADS and the ADS mutant enzymes with structurally distinct starter substrates. A–H, the TLC-based analysis of radiolabeled products from malonyl-CoA (1) and hexanoyl-CoA (10) (A), octanoyl-CoA (2) (B), decanoyl-CoA (11) (C), lauroyl-CoA (12) (D), myristyl-CoA (13) (E), palmitoyl-CoA (14) (F), N-methylanthraniloyl-CoA (3) (G), and p-coumaroyl-CoA (7) (H) by the ADS and ADS mutant enzymes. I, HPLC elution profiles of enzyme reaction products obtained by ADS and the boiled ADS reactions with acetyl-CoA (9) and 1 as the substrates.