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dCDP formation by WT-α2 and E52Q/F3Y122•-β2 (0.91 Y122•/β2) in the presence of CDP (blue), CDP/ATP (red), or CDP/ATP and reductant TR/TRR/NADPH (green). The reaction mixture contained 20 μm of 1:1 subunits in 30 μl. In these experiments α2 was pre-reduced. Each point represents the average of two independent trials.
