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. 2017 Apr 7;292(22):9283–9293. doi: 10.1074/jbc.M116.773739

Figure 5.

Figure 5.

Increased expression of WNK1 and WNK3 may contribute to elevated SGK1 activity in Akt3−/− macrophages. A, expression of WNK1, WNK2, WNK3, and WNK4 in WT (n = 5) and Akt3−/− MPMs transfected with control (n = 5) or Akt3 cDNA (n = 5). B, WNK1 and WNK3 expression in WT or Akt3−/− macrophages treated with control (Ctl) or WNK1- or WNK3-specific siRNA (n = 4). C, phospho-NDRG1 and NDRG1 expression in Akt3−/− MPMs treated with control, WNK1, or WNK3 siRNA. SGK1 activity was calculated as phospho-NDRG1/NDRG1 ratio. n = 4. *, p < 0.05 versus control. D, pinocytosis of Lucifer yellow dye by WT, Akt3−/− (Ctl), and Akt3−/− MPMs treated with WNK1 siRNA (W1i) or WNK3 siRNA (W3i) (n = 6). Data represent means ± S.E. *, p < 0.05. Data are representative of at least three independent experiments and are quantified from at least three independent experiments.