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. 2017 Apr 10;292(22):9294–9304. doi: 10.1074/jbc.M116.773069

Figure 5.

Figure 5.

Top-down MS of E73Q and E73A mVDAC1 photolabeled with cholesterol analogues. A, deconvoluted spectra of E73Q mVDAC1 photolabeled with LKM38 and KK174. For LKM38, the two peaks represent unlabeled and singly labeled E73Q at 32,153 and 32,550 Da, respectively. For KK174, the two peaks represent unlabeled and singly labeled E73Q at 32,153 and 32,493 Da, respectively. B, same as A for E73A mVDAC1. For LKM38, the two peaks represent unlabeled and singly labeled E73A at 32,097 and 32,493 Da, respectively. For KK174, the two peaks represent unlabeled and singly labeled E73A at 32,097 and 32,477 Da, respectively. C, HCD fragment ion assignments obtained by isolating the LKM38- and KK174-E73Q photolabeled species, respectively. The gray lines represent b and y ions that do not contain a ligand adduct. The black lines represent b ions that contain ligand adduct identified by searching against a database in which the ligand mass was added to the N-terminal methionine. The red lines represent y ions that contain ligand adduct identified by searching against a database in which the ligand mass was added to the C-terminal alanine. D, same as C for E73A mVDAC1.