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Formation of a long-lived reactive intermediate for KK174 but not LKM38. SDS-PAGE of mVDAC1 photolabeled with 30 μm KK174 and LKM38. KK174 and LKM38 were UV irradiated for 1 min in the presence of mVDAC1 (UV Ligand + Protein), or in the absence of mVDAC1 followed by immediate mixing with mVDAC1 (UV Ligand). The gel bands show fluorescence signal with TAMRA (left) or SYPRO Ruby (right).
