Figure 6.

GIMAP6 expression and NF-κB activation in PMA/ionomycin-treated Jurkat cells. A, up-regulation of GIMAP6 in Jurkat T cells was detected after treatment with PMA and/or ionomycin, and the effect of PMA induction was suppressed by the AP-1 inhibitor SP100030. As a control, Jurkat T cells were treated with an equal concentration of DMSO. RNA was harvested at 24 h for quantitative PCR. The expression levels of GIMAP6 relative to HPRT1 are presented as the log2 -fold. Statistical calculation was determined using Student's t test. Significance relative to the DMSO group is indicated as ***, p < 0.001 and **, p < 0.01. Significance relative to the PMA group is indicated as ###, p < 0.001 and #, p < 0.05. SP indicates SP100030 at the indicated concentration. B, cell lysates were also harvested at 48 h for immunoblot analysis. The relative -fold change was calculated as GIMAP6/β-tubulin, and the intensity in Mock was taken as 1. C, reduction of p65 phosphorylation (Ser-536) under the PMA/ionomycin treatment condition is associated with GIMAP6 knockdown. Three Jurkat-derived cells (Ctl, KD1, and KD2) were harvested at the indicated time points after PMA/ionomycin induction.