Figure 3.

p53C aggregation in the presence of nucleic acids and rNTPs at 37 °C followed by LS at 400 nm. A, relative aggregation of p53C (4.5 μm) in the absence/presence of different RNAs after 1.5 h of incubation. B, relative aggregation of p53C in the absence/presence of single/double-stranded DNA and rNTPs after 1.5 h of incubation. C, reversal of the RNA effect on p53C aggregation by RNase treatment (5 μm RNase A) before incubation with p53C. All measurements were performed at least in triplicate, and the error bars indicate S.D. AU, arbitrary units.