Figure 7.

Characterization of p53C aggregation with ThT binding, dot-blot assay with A11 antibody and seeding experiment. A, time-resolved ThT fluorescence of increasing concentrations of p53C at 37 °C. The final ThT concentration was 115 μm. B, amyloid oligomer specific (A11) dot-blot analysis of p53C aggregates formed in the absence of RNA (top) or at the 1:50 (middle) and at 1:8 RNA to protein (bottom) ratios of DHFR mRNA (left) and dom V rRNA (right). C, time course of aggregation of p53C at 37 °C after seeding the reaction with 5% (v/v) supernatant from the end products of p53C aggregation without or with GFP+ mRNA in low and high RNA:protein ratios (see “Experimental Procedures” for details). AU, arbitrary units.