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. 2017 Apr 13;292(22):9420–9430. doi: 10.1074/jbc.M117.783787

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — Overexpression of TAZ in the immortalized prostate epithelial cells promotes EMT, cell migration, and anchorage-independent growth. A, the representative phase-contrast images and rhodamine-conjugated phalloidin staining images of vector control RWPE1 cells, TAZ^S89A overexpressing RWPE1 cells, and TAZ^S89A-S51A overexpressing RWPE1 cells were shown. B, Transwell assay of the vector control, TAZ^S89A, and TAZ^S89A-S51A RWPE1 cells. Representative images of the Transwell migration assays were shown. Relative migration ability was normalized to the vector control group. *, p < 0.05 by the Student's t test. C, soft agar assay of the vector control, TAZ^S89A, and TAZ^S89A-S51A RWPE1 cells. Representative images of soft agar colonies were shown. Clone numbers of each well were counted. *, p < 0.05 by the Student's t test. D, Western blotting analysis of the EMT markers (FN1, N-cadherin, vimentin, β-catenin, and E-cadherin) in the vector control, TAZ^S89A, and TAZ^S89A-S51A RWPE1 cells were shown. Quantitative analysis of Western blotting was indicated. Error bars, standard deviation.