Figure 3.

Stimulation of the rate of glucose consumption by nanomolar NO. A, astrocytes expressing the glucose sensor were exposed for 3 min to 2 μm NO (n = 5 cells, representative of four experiments). B, astrocytes expressing the glucose sensor were first transiently depleted of glucose for calibration purposes, and then the rate of glucose consumption was estimated in the presence of the GLUT1 blocker cytochalasin B (20 μm) before and after addition of 10 nm NO to the superfusate. After washout, the same protocol was repeated at 100 nm NO. Data are shown for three cells, which responded to 100 nm NO with varying degrees of glucose consumption stimulation.