Table 3.

Analysis of variance examining the effects of temperature (development and adult acclimation) on flies developed and adult acclimated under three different treatments 16–30/16–30°C, 16–30/25°C, and 25/16–30°C on CTmax for northern and southern populations of D. melanogaster and adult acclimated for 23 days.

treatment	fixed effects	d.f	MS	F
16–30/16–30 versus 16–30/25	population	1	0.229	1.069
	temperature (acclimation)	1	12.482	58.210***
	treatment	1	5.943	27.715***
	run	4	2.106	9.823***
	scorer	3	0.580	2.704*
	temperature × treatment	1	2.548	11.883***
	temperature × run	4	1.444	6.735***
	population × scorer	3	0.728	3.395*
	error	685	0.214
16–30/16–30 versus 25/16–30	population	1	0.299	1.186
	temperature	1	6.555	26.019***
	treatment	1	8.327	33.055***
	run	4	2.303	9.142***
	scorer	3	0.472	1.874
	temperature × treatment	1	6.378	25.315***
	temperature × run	4	1.648	6.541***
	temperature × scorer	3	1.030	4.087**
	error	682	0.252