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. 2017 May 31;14(130):20170131. doi: 10.1098/rsif.2017.0131

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© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — GM-CSF produced by dNK cells after activation of KIR2DS1 stimulates trophoblast migration. Trophoblast migration measured with a chemical gradient of supernatants from dNK cells after (a) KIR2DS1+ dNK cells activated by mAb, EB6 (supernatant I), anti-GM-CSF neutralizing antibody (supernatant II) or negative control (supernatant III). (b) Trophoblast cells migrate with increased directionality towards supernatants produced by activated KIR2DS1+ dNK cells. Directed migration is reduced when anti-GM-CSF neutralizing antibody is added. (c) A large fraction of cells migrate towards increasing concentrations of supernatants produced by activated KIR2DS1+ dNK cells. *p < 0.05 calculated using one-way analysis of variance (ANOVA) and Dunn's multiple comparisons test. (Online version in colour.)