Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 18;8:15428. doi: 10.1038/ncomms15428

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Thermograms (upper panels) in the presence of increasing concentrations of crowders: (a) PEG 20, (b) dextran 40, (c) Ficoll 70 and (d) Ficoll 400. All curves were obtained starting from solutions of 10 μM Yfh1 in a 10 mM HEPES buffer at pH 7.0 by monitoring the CD intensity at 220 nm as a function of temperature in the temperature range 275 K to 343 °C in the presence of varying concentrations of crowders. Stability curves (lower panels) of Yfh1 corresponding to the thermograms (upper panels): (a) PEG 20, (b) dextran 40, (c) Ficoll 70 and (d) Ficoll 400. The curve corresponding to 20% w/v PEG 20 is represented as a dashed line because the fitting was extrapolated from those at lower concentrations; this is due to the observation that the thermogram (upper (a) panel) shows only very upward curvature at low temperatures.