Figure 2. Lhx1/5 DKO mutants display motor deficits.

(a) Scatter plots showing differences in retention time before falling from an accelerating rotarod from 4 to 40 r.p.m. in 5 min (left) or 2 min (middle) and stationary rotarod at 15 r.p.m. (right) between the controls (n=22 mice) and the DKO mutants (n=14 mice). Bars in the scatter plots represent the mean values. (b) Graphs showing differences in time to complete running on an elevated balance beam (left) and number of missteps (right) between the controls and the DKO mutants. Brackets show the number of the control and the DKO mutant mice tested; t-test; **P<0.01 and ***P<0.001. (c) Representative photos showing the walking behaviour of a control (upper panels) and a DKO mutant (lower panels). At least three mice per group were tested for their walking behaviour. (d) Example of paw prints acquired during footprint analysis. The DKO mutant footprint showed some tottering steps (red bracket) during initiation of locomotion. In all, 21 control mice and 17 mutant mice were analysed.