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. 2017 May 18;8:15079. doi: 10.1038/ncomms15079

Figure 7. C-terminus of actin-bundling module of Espin is required for proper F-actin localization.

Figure 7

(a) Schematic representation of Espin-DsRed and jerker-Espin-DsRed. The nucleotide in blue (marked by asterisk) was deleted and resulted in a frameshift mutation in C-terminus of jerker-Espin. The resulting jerker-Espin-DsRed had a shorter and mutated actin-bundling module (ABM*). (b–e) Central focal planes of the PC proximal dendrites from P14 control (b) or DKO mutant (c–e) co-transfected with EGFP-Actin and pDsRed (b,c) or Espin-DsRed (d) or jerker-Espin-DsRed (e). Scale bars, 10 μm. (f–i) Fluorescence intensity profile plots (across the yellow doted lines) reveal the distribution of F-actin in the dendritic shafts of the proximal dendrites of the transfected PCs, respectively. Areas shaded in cyan represent the dendritic shafts. Each profile plot was sampled from 10 different PCs from 4 to 5 different mice and the curves represent mean±s.e.m. Note that the F-actin localization in the mutant PCs restored to the controls' pattern after transfection of Espin-DsRed.