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. 2017 May 18;8:15346. doi: 10.1038/ncomms15346

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Copyright © 2017, The Author(s)

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Western blots (a,c,e) and densitometric analysis (b,d,f) of oocytes from 1 month- and >1 year old mice for securin during MII arrest (14 h post release from GV, 50 oocytes per lane), GV arrest (20 oocytes per lane) and MI (8 h post release from GV, 20 oocytes per lane). Actin was used as a loading control. Results are mean±s.e.m. ^NSP>0.05 and **P<0.01. P values were calculated with one-sided Student's t-test. All western blots were repeated three times using oocytes from two to six mice per experimental group.