Table 2. Kinetic parameters of the DBAT mutants against DT.
| DBAT | DBATG38R | DBATF301V | DBATG38R/F301V | |
|---|---|---|---|---|
| Vmax (μM min−1) | 0.204 (±0.004) | 0.499 (±0.029)*** | 0.242 (±0.008)** | 0.918 (±0.096)*** |
| Km (mM) | 0.546 (±0.029) | 0.368 (±0.061)* | 0.178 (±0.017)*** | 0.424 (±0.133) |
| kcat (s−1)(× 10−3) | 0.334 (±0.001) | 0.815 (±0.005)*** | 0.396 (±0.013)** | 1.499 (±0.157)*** |
| kcat/Km (M−1 s−1) | 0.611 (±0.243) | 2.218 (±0.787)* | 2.22 (±0.771)* | 3.539 (±0.118)*** |
The data represent the means±s.d., n=3. *P<0.05 versus DBAT (Control), **P<0.01 versus DBAT (Control), ***P<0.001 versus DBAT (Control) (Student’s t-test).