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. 2017 May 10;9(5):105. doi: 10.3390/v9050105

Figure 2.

Figure 2

AME inhibits HIV integration. (A–C) SupT1 cells were infected with vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped NL4-3Luc R-E- viruses with the treatment of DMSO, 4 nM EFV, 25 nM RAL, 60 µM AOH, or AME of indicated concentrations as described in the Materials and Methods section. DNA was extracted from infected cells at 3 h, 8 h, and 24 h post-infection. HIV early reverse transcription products (A), integrated DNA (B), and 2-LTR circles (C) were analyzed by RQ-PCR using the corresponding primers mentioned in the Materials and Methods section; (D) The activity of integrase was measured by an ELISA-based in vitro assay in the treatment of DMSO, 100 µM AME, and 100 nM RAL. * p < 0.05.