Figure 5.

AME does not disturb the classical nuclear transport pathway. (A) HeLa cells were transfected with Rev-HA and treated with DMSO, ivermectin or AME. One day post-infection, cells were fixed and stained with mouse anti-HA antibody followed by TRITC-conjugated goat anti-mouse secondary antibody. The nuclei were stained with 4′6′-diamidino-2-phenylindole (DAPI). Cells were analyzed by confocal microscopy (with a 60× objective lens); (B) HEK293T cells were treated with DMSO, ivermectin, or AME. One day post-transfection, cells were fixed and stained with mouse anti-SV40 T antigen antibody followed by FITC-conjugated goat anti-mouse secondary antibody, and the nuclei were stained with 4′6′-diamidino-2-phenylindole (DAPI). Cells were examined by confocal microscopy. More than 25 cells per condition spanning three independent experiments were included for each panel.