Figure 2.

ADE of infection in SK6-CD16 cells. Sera from TRIP/JEV.prME-immunized, JEV Laos- and JEV Nakayama-infected pigs were tested for ADE activity in the porcine kidney cell line SK6 expressing CD16. ADE of infection was tested as described in Figure 1 using (a) JEV G1 Laos; (b) JEV G3 Nakayama and (c) JEV G5/G3, representing a chimeric virus expressing a G5 prM/E. The percentage of infected cells was determined after 24 h. Statistical significance was calculated using a two-way ANOVA followed by Dunnets’s multiple comparison. The results are representative of triplicate cultures repeated in two independent experiments. * p < 0.05, ** p < 0.002, *** p < 0.001.