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. 2017 May 22;9(5):124. doi: 10.3390/v9050124

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TRIP/JEV.prME vaccine or previous JEV infection induce protection but do not prevent organ infection. Groups of three pigs were either unvaccinated/infected (negative control; Neg. CTRL, crosses), immunized by previous infection with JEV Laos (squares) or vaccinated with TRIP/JEV.prME (circles), and then challenge infected with JEV G3 Nakayama. At 10 days p.i., the animals were euthanized and organ samples tested for viral RNA by RTqPCR. (a) viral RNA load in pigs from the Neg. CTRL group; (b) viral RNA load from the JEV Laos pre-infected group; (c) viral RNA load from the TRIP/JEVprME-vaccinated group.