Table 2.

Primer and probe sets employed for JEV reverse transcription-quantitative PCR (RT-qPCR).

Specificity		Primer and Probe Sequence (5′-3′)	Concentration (nM)
3′ NTR JEV	forward	GGTGTAAGGACTAGAGGTTAGAGG	200
	reverse	ATTCCCAGGTGTCAATATGCTGTT	200
	probe	FAM-CCCGTGGAAACAACATCATGCGGC-BHQ-1	100
JEV G1 Laos	forward	GACAGGATAAAGTCATGTGCGT	200
	reverse	CCTGACGTTGGTCTTTCAAC	200
	probe	FAM-CCGTCTCGGAAGCAGGTCCC-BHQ-1	100
JEV G3 Nakayama	forward	CAGGGTCATCTAGTGTGATTTAAGG	1600
	reverse	CAGTCCTCCTGGGACTGAGA	1600
	probe	FAM-TGCTGGCCTGACTCCATATGCA-BHQ-1	200

Infectious virus quantification was determined by titration on Vero cells as previously described [23].