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. 2017 May 12;49(5):e328. doi: 10.1038/emm.2017.40

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of BFP-2 treatment on ALP, osteocalcin, CD44 and CD51 expression levels. BMSCs were treated with 1 μg ml⁻¹ of BFP-2 or1 μg ml⁻¹ of BMP-7 (as a positive control). After 24 h, immunofluorescence analyses were carried out using (a) anti-ALP (green), (b) anti-osteocalcin (green) and (c) anti-CD44 (red) antibodies. (d) CD44 and CD51 expression was measured by flow cytometry (green arrow: ODM only, pink arrow: ODM plus BFP-2). The data are representative of four independent experiments. Magnification, × 400.