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. 2017 May 12;49(5):e329. doi: 10.1038/emm.2017.41

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Suppressing TMEM119 expression represses the proliferation of osteosarcoma cells. (a) TMEM119 expression in five osteosarcoma cell lines was analyzed by real-time PCR (left panel) and western blotting (right panel) using GAPDH as the internal control. The experiments were repeated at least three times with similar results. (b) TMEM119 expression in U2OS and MG63 cells was analyzed by real-time PCR (left panel) and western blotting (right panel). The experiments were repeated at least three times with similar results. (c) Cell proliferation was detected in siRNA-transfected U2OS and MG63 cells by the CCK-8 assay. ***P<0.001.