Figure 2.

Protein expression of Car4 and Car9 during wound healing. Skin excision wounds were generated in WT mice as described in the Methods section. Normal skin and skin wound samples were collected from unwounded mice and from mice killed at various time points after wounding. The skin samples were processed for IHC analysis, and CA IV and CA IX were detected using specific antibodies as described in the Supplementary Methods. The results obtained at all studied time points are shown. CA IV protein expression started to accumulate in the early granulation tissue at day 5 of healing, and positive expression remained in the wound until day 10, after which the expression disappeared. CA IV protein was expressed mainly by the cells in granulation tissue, with especially strong expression just beneath the migrating epidermis (arrows). CA IX protein expression, in turn, was already detected in the skin wound at day 2 and remained elevated throughout the wound healing process. Animal numbers: all time points: n=6 mice with two wounds; total n=12.