Figure 2.

GABRP promotes migration and invasion of SK-OV-3 cells through ERK pathway. SK-OV-3 cells were transfected with EGFP and GABRP expression constructs (a) or with siNC and siGABRP (b). After transfection, the effects on migration were determined using transwell assays, and the effects on invasion were determined using Matrigel-based invasion assays. Representative images of migrated or invaded cells are shown (× 100 magnification). The amounts of migrated or invaded cells were quantified by measuring the absorbance of extracts from cell stains at 595 nm (a, b). After treatment with the MEK inhibitor U0129, the migration and invasion of SK-OV-3 cells were determined using transwell and Matrigel-based invasion assays, respectively (c). Immunoblot analysis of total ERK1/2 and phosphorylated ERK1/2 proteins was performed in cells treated with expression plasmid constructs, siRNAs or MEK inhibitor (d). A representative immunoblot of total ERK1/2 and phosphorylated ERK1/2 proteins (upper panel of d), and quantification of phosphorylated ERK1/2 relative to total ERK1/2 (lower panel of d), are shown. All data shown are means±s.d.'s for triplicate measurements. Statistical analysis was performed with t-tests (*P<0.05, ^**P<0.01, ^***P<0.001). siGABRP, GABRP siRNA; siNC, non-targeting control siRNA.