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. 2017 May 19;8:15242. doi: 10.1038/ncomms15242

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(a) PT depends from the WN₃ 2 dose delivered by IP injection. Dose/response curve of WN₃ 2 was measured 6 h after the second injection of 1 and 2 mg kg⁻¹ of WN₃ 2 and compared to vehicle-treated mice (two IP injections, n=4). (b) PT depends on the delay after treatment. WN₃ 2 (2 mg kg⁻¹) was administered twice with a 24-h interval by IP injection and PT was measured 3, 6 and 12 h (n=4–6/group) after the second treatment and compared to vehicle-treated mice. Statistical differences identified with protected least significant difference (PLSD) Fischer test were indicated as: *P<0.05, **P<0.01 as compared with vehicle-treated mice. Error bars indicate s.e.m. values.