Figure 7. Effects of in vivo click reaction on WN₃ (2) distribution and renal excretion.

(a) WN₃ 2 concentration was measured in the plasma, urine and liver of mice treated with a single dose of WN₃ 2 (2.0 mg kg⁻¹, 5.7 μmol kg⁻¹, 1 eq) by IP injection (n=3). (b–d) Analyses of free and clicked WN₃ 2 and BCN-peg₆-OH 9 after single IP injection of WN₃ 2 (2.0 mg kg⁻¹, 5.7 μmol kg⁻¹, 1 eq) followed 5 min later by SC injections of BCN-peg₆-OH 9 (52 mg kg⁻¹, 114.4 μmol kg⁻¹, 20 eq) (n=3). (a–d) Samples collection have been performed 50–60 min after the last injection. Note that plasma (red) and urine (blue) concentrations of all substrates were reported in nmol ml⁻¹ whereas liver (green) levels were shown in nmol g⁻¹. <LOD (limit of detection), below the detection limit. ***P<0.001 as compared to WN₃ 2 in respective compartments shown in ‘No click reaction' panel. ^###P<0.001 as compared to liver and plasma measures of the corresponding compound. Error bars indicate s.e.m.