Table 3.
Ion channel gene expression changes in slo and Shab
|
Shab vs. Wild Type |
slo vs. Wild Type |
||||
|---|---|---|---|---|---|
| Ion channel | Change | FPKM (CI) | Ion channel | Change | FPKM with CI |
| CG42260, CNG family cation channel | 2.64-fold increase | CS = 4.58 (2.60–6.56) | CG42260, CNG family cation channel | 2.67-fold increase | CS = 4.58 (2.60–6.56) |
| Shab = 12.09 (7.95–16.22) | slo = 12.21 (8.05–16.38) | ||||
| slo, Ca2+-activated potassium channel | 1.68-fold increase | CS = 7.21 (4.56–9.85) | CG8713, two-pore potassium channel | 13.94-fold increase | CS = 0.92 (0–2.51) |
| Shab = 12.07 (7.83–16.31) | slo = 12.80 (5.67–19.92) | ||||
| cngl, CNG family cation channel | 1.57-fold increase | CS = 11.06 (7.35–14.76) | CG42732, Ca2+-activated potassium channel | 1.61-fold increase | CS = 9.38 (5.96–12.79) |
| Shab = 16.14 (10.79–21.48) | slo = 15.12 (10.19–20.05) | ||||
| Ih, hyperpolarization-activated cation channel | 1.46-fold increase | CS = 25.88 (16.59–35.18) | CG7589, ligand-gated chloride channel | 5.96-fold decrease | CS = 1.56 (0–3.36) |
| Shab = 38.04 (24.53–51.55) | slo = 0.26 (0–0.62) | ||||
| cac, voltage-gated calcium channel, Cav2 homolog | 1.40-fold increase | CS = 15.30 (10.28–20.31) | rpk, amiloride-sensitive sodium channel | 2.04-fold decrease | CS = 2.32 (0.37–4.30) |
| Shab = 21.40 (14.62–28.19) | slo = 1.02 (0–2.21) | ||||
| CG11155, ligand-gated cation channel | 1.32-fold increase | CS = 84.21 (49.07–119.21) | |||
| slo = 109.19 (66.52–151.85) | |||||
| NtR, ligand-gated cation channel | 1.74-fold decrease | CS = 7.19 (2.96–11.42) | |||
| slo = 4.10 (1.22–6.99) | |||||
Libraries were prepared from third instar larval brains of Canton S (CS) wild type, slo1, and Shab3, and resulting profiles were compared using Cuffdiff (Cufflinks 2.1.1) to extract statistically significant (P < 0.05) differences to identify ion channel genes. Three replicates were done for each genotype. Fragments per kilobase of transcript per million mapped reads (FPKM) are shown with the confidence intervals (CI) used for differential expression testing to give an estimate of expression levels and variability. Note that only CG42260, slo, and CG42732 met the more stringent Q < 0.05 cutoff.