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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Exp Neurol. 2017 Mar 21;292:179–192. doi: 10.1016/j.expneurol.2017.03.010

Figure 4.

Figure 4

A. Electron photomicrograph showing immunoperoxidase labeling of HA-α2A-adrenoceptor (HA-αAR-t) and immunogold-silver labeling (arrows) of CB1r in separate axon terminals in the mPFC of an HA epitope-tagged HA-α2A-adrenoceptor knock-in mouse. B. An immunoperoxidase-labeled axon terminal contains dense immunoreactivity for HA-α2A-adrenoceptor and immunogold-silver labeling for CB1 receptor (HA-αAR+ CB1r-t). The dually labeled terminal forms an asymmetric type synapse (zigzag arrows) with an unlabeled dendrite (ud) that contains a mitochondria (m) and is apposed by an unlabeled terminal (ut). C. A dually labeled HA -α-adrenoceptor +CB1r-t axon terminal forms a symmetric type synapse (arrowheads) with an unlabeled dendrite (ud) containing a mitochondria (m). D. A densely peroxidase labeled HA-αAR-t is shown contacting an unlabeled axon terminal (ut1) that contacts another HA-αAR-t that in turn contacts a CB1 receptor-labeled dendrite (CB1r-d) containing several mitochondria (m). Arrows point to immunogold-silver labeled particles. The CB1r-t also receives a synapse from another unlabeled terminal (ut2). E. HA-αAR-t is shown contacting a CB1r-d with several mitochondria (m). F. A HA-αAR-t forms an asymmetric synapse (zigzag arrows) with a CB1r-d and is directly apposed by an unlabeled terminal (ut). m: mitochondria; ut: unlabeled terminal. Scale bars, 0.5 μm.