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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Exp Neurol. 2017 Mar 21;292:179–192. doi: 10.1016/j.expneurol.2017.03.010

Figure 5.

Figure 5

Ultrastructural evidence for co-localization of HA-α-adrenoceptor and CB1 receptor in dendrites. A. An immunoperoxidase-labeled dendrite contains HA-α2A-adrenoceptor immunoreactivity as well as immunogold-silver labeling for CB1 receptor (HA-αAR+CB1r-d). This dendrite is directly contacted by an unlabeled terminal (ut). B. A dually labeled HA-αAR+CB1r dendrite receives an asymmetric type synapse (zigzag arrows) from an unlabeled terminal (ut1) and a symmetric type synapse (arrowheads) from ut2 and ut3. A fourth unlabeled terminal (ut4) directly contacts the HA-αAR+CB1r dendrite. C. A dually labeled HA-αAR+CB1r-d receives two symmetric type synapses (arrowheads) from ut1 and ut2. D. A CB1 receptor labeled axon terminal (CB1r-t) forms a symmetric type synapse (arrowheads) with HA-α-adrenoceptor labeled dendrite (HA-αAR-d). The same HA-αAR-d is contacted by an unlabeled terminal (ut). E. A CB1r-t forms an asymmetric type synapse (zigzag arrows) with an unlabeled dendrite (uD) and a HA-αAR-d. m: mitochondria; ut: unlabeled terminal. Scale bars, 0.5 μm.