Fig. 2.

Feeding Angptl4^−/− mice a diet rich in saturated, but not trans- or cis-unsaturated, FAs causes marked elevation of acute phase proteins. Angptl4^−/− mice were fed the trans, unsaturated, or saturated fat diet for 7 weeks. A: Weekly circulating levels of SAA. B: Relative hepatic mRNA level of Saa2. C: Plasma levels of haptoglobin. D: Relative hepatic mRNA level of Hp. E: Plasma levels of lipocalin-2. F: Relative hepatic mRNA level of Lcn2. mRNA expression levels were normalized against 36b4. Please note that the Angptl4^−/− mice fed the saturated fat diet were euthanized at different time points due to the implementation of humane endpoints. Error bars represent SEM. Asterisks indicate significantly different relative to trans fat group (*P < 0.05).