Fig. 4.

mL-STL is a liver-specific and male-dominant protein. Different tissues excised from WT male and female mice were subject to Northern blot (top) and Western blot (bottom) analyses. For Northern blot analysis, total RNA (20 µg/lane) was electrophoresed in a 1% formaldehyde agarose gel, transferred onto a N⁺-nylon membrane, and hybridized with a DIG-labeled mL-STL cDNA probe. The integrity and uniformity of the RNA are indicated by 28S and 18S on the formaldehyde agarose gel. M, DIG-labeled RNA molecular mass marker I (0.39–6.9 kb). For Western blot analysis, cytosolic proteins (20 µg/lane) were electrophoresed in a 12% SDS-PAGE gel, transferred onto a PVDF nylon membrane, and incubated with an mL-STL antiserum (1:5,000) and a mouse monoclonal anti-β-actin (1:30,000) antibody. β-actin was used as a loading control. M, Invitrogen Benchmark prestained protein ladder (6–180 kDa). The experiment was repeated with three different batches of animals, and the data shown are representative of Northern (top) and Western (bottom) blots.