Fig. 1.

Kidney triglycerides increased in both male and female mice after an overnight fast. A–E: Male and female mice were either fasted or given food ad libitum for 16 h. Blood was drawn to measure glucose and plasma lipids. Lipids were extracted for intracellular triglyceride measurements. A: Plasma glucose. B: Plasma triglycerides. C: Plasma total cholesterol. D: Plasma NEFAs. E: Kidney triglyceride content. F: Kidneys from male mice were formalin fixed and sectioned for oil-red O staining. Scale bar is representative of 100 μM at 20× magnification. N = 4–6/group. *P < 0.05. Results are presented as mean ± SD. *Results compared by unpaired Student’s t-test within each sex group, no significant differences were found between male and female groups using the two-way ANOVA Tukey’s multiple comparison test.