Fig. 2.

DGAT1 p.L105P protein is found at reduced levels in cells. A: DGAT1 transcript levels are similar in control and patient fibroblasts. Relative mRNA levels determined by quantitative real-time PCR are shown here. B: Immunoblot shows less endogenous DGAT1 protein in mutant fibroblast lysate than in controls. C: FLAG-tagged wild-type and p.L105P DGAT proteins were expressed in SUM159 cells. The cells were treated with cycloheximide and lysed at different times. Immunoblot shows wild-type p.L105P DGAT1 protein may be degraded more rapidly compared with wild-type. D: Quantification of data shown in (C) and a second independent experiment. Statistical significance was determined by linear regression; *P < 0.05.