Table 1. POLB splice variants in TK6 cells characterized by transcript cloning.
| POLB splice variants | PTC | Putative protein length (amino acids) | Splice variant frequency (%)a | Splice variant frequency relative to WT frequency (%)b | ||
|---|---|---|---|---|---|---|
| Untreated TK6 | Emetine-treated TK6 | Untreated TK6 | Emetine-treated TK6 | |||
| Intron retention | ||||||
| Σ exon α | No | 370 | 4.6 | 4.7 | 7.7 | 9.5 |
| Σ exon β | Yes | 193 | 0 | 11.8 | 0 | 23.8 |
| Σ exons α and β | Yes | 228 | 0 | 2.4 | 0 | 4.8 |
| WT | No | 335 | 59.8 | 49.4 | 100 | 100 |
| Exon skipping | ||||||
| Δ exon 2 | Yes | 26 | 13.8 | 9.4 | 23.1 | 19.0 |
| Δ exons 2 and 3 | Yes | 28 | 0 | 1.2 | 0 | 2.4 |
| Δ exons 2 and 11 | Yes | 26 | 0 | 1.2 | 0 | 2.4 |
| Δ exons 2, 3 and 11 | Yes | 28 | 0 | 4.7 | 0 | 9.5 |
| Δ exons 2, 11–13 | Yes | 26 | 1.1 | 0 | 1.9 | 0 |
| Δ exons 2–13 | No | 51 | 0 | 1.2 | 0 | 2.4 |
| Δ exon 4 | No | 310 | 1.1 | 0 | 1.9 | 0 |
| Δ exons 4 and 5 | Yes | 96 | 0 | 1.2 | 0 | 2.4 |
| Δ exons 4–11 | No | 161 | 2.3 | 0 | 3.8 | 0 |
| Δ exons 4–11 and 13 | No | 93 | 0 | 1.2 | 0 | 2.4 |
| Δ exons 4–13 | No | 115 | 0 | 2.4 | 0 | 4.8 |
| Δ exon 11 | No | 306 | 10.3 | 5.9 | 17.3 | 11.9 |
| Δ exons 11–13 | No | 261 | 2.3 | 2.4 | 3.8 | 4.8 |
| Δ exons 12 and 13 | No | 290 | 4.6 | 0 | 7.7 | 0 |
aThis value is calculated according to the formula frequency = (number of observations of the splice variant/total number of transcripts analyzed) × 100.
bThis value is calculated according to the formula frequency = (number of observations of the splice variant/number of observations of the WT) × 100.