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. 2004 Dec 15;32(22):6575–6584. doi: 10.1093/nar/gkh991

Figure 4.

Figure 4

Telomere shortening in ΔtbKU80 trypanosomes. Genomic DNA from wild-type cells, tbKU80 single allele knockout cells (tbKU80+/−), tbKU80-deficient cells (ΔtbKU80) and from a tbKU80-deficient cell line, which expressed an ectopic copy of GFP–tbKU80 (ΔtbKU80 + GFP–tbKU80) was prepared every other week for a period of 8 weeks. The DNA was digested with AluI, HinfI and RsaI, and separated by agarose gel electrophoresis, Southern-blotted and probed with a radiolabeled telomeric (TTAGGG)27 probe. Distinguishable bands of ΔtbKU80 telomeric DNA were used to estimate telomere shortening rates during 8 weeks.