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Primer sets for PCR Amplification, Pyrosequencing, and Fragment Analysis
| Mutation | Primer name | Primer sequence (5’-3’) | Amplicon length (bp) |
|---|---|---|---|
| JAK2 (V617F) | JAK2-forward | TATGATGAGCAAGCTTTCTCACAAG | 102 |
| JAK2-reverse | biotin-AGAAAGGCATTAGAAAGCCTGTAGTT | ||
| JAK2-sequencing | GGTTTTAAATTATGGAGTATGT | ||
| MPL (W515K/L) | MPL-forward | CCGCTCTGCATCTAGTGCT | 79.0 |
| MPL-reverse | biotin-CTGTAGTGTGCAGGAAACTG | ||
| MPL-sequencing | TGCTGCTGCTGAGGT | ||
| CALR exon 9 | CALR-forward | 6-FAM-GGCAAGGCCCTGAGGTGT | - |
| CALR-reverse | GGCCTCAGTCCAGCCCTG |
