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. 2004 Dec 21;32(22):e187. doi: 10.1093/nar/gnh176

Figure 2.

Figure 2

Electric pulse-triggered transfection on a plasmid-loaded electrode. HEK293 cells and PMEF were cultured on the pEGFP-loaded electrode for 24 h, and then treated with a single electric pulse for 10 ms duration at the field strengths of 75 and 100 V/cm, respectively. Primary hippocampal neurons were cultured on the pEGFP/pDsRed-loaded electrode for 72 h, and then treated with a single electric pulse for 10 ms duration at 100 V/cm. Two days after electroporation, cells were observed for assessing EGFP and DsRed expression. (A) Fluorescence image of EGFP-expressing HEK293 cells treated with an electric pulse. (B) No EGFP was expressed by HEK293 on a plasmid-loaded electrode when an electric pulse had not been applied. (C) Phase-contrast image of transfected PMEF. (D) Fluorescence image of the same sight as in (C). (E) Phase-contrast image of transfected hippocampal neurons. (F and G) Fluorescence images of the same sight as in (E) acquired using an interference filter for (F) EGFP and (G) DsRed. (H) Overlay image of (F) and (G). Scale bar = 200 μm.