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Influence of the molecular weight of PEI on the transfection efficiency. Transfection efficiency is plotted as a function of (A) the molecular weight of PEI and (B) the amount of plasmid DNA released upon electric pulsing at the field strength of 75 V/cm with a pulse number of 1–4 and a pulse duration of 10 ms. Molecular weight of PEI: (open circles) 800, (solid circles) 25 000, and (open squares) 750 000. The data are expressed as a mean ± SD for five independent experiments.
