Table 1.
Oligonucleotide primers used in this study.
| Primer | Sequence(5′-3′) | Restriction sites | Ligated vector | Template |
|---|---|---|---|---|
| 27F | AGAGTTTGATCCTGGCTCAG | |||
| 1492R | GGTTACCTTGTTACGACTT | |||
| P1 | TCATTGCGCCAATCTTTACAAT | L. amylovorus CGMCC 11056 | ||
| P2 | CAGATTTGCGAGATTTTCATG | |||
| P3 | GATAAAATTAATCTTTCCAAT | L. acidophilus CCTCC AB2010208 | ||
| P4 | CAGATTTGCGAGACTTTCATG | |||
| P5 | CCGATGATTAAAAATTTTTCCATT | L. farciminis CCTCC AB2016237 | ||
| P6 | CGCTCAAAACGTGTGCCCGT | |||
| P7 | ACTTTGCGCCCCGCCGCTTGT | L. fermentum CCTCC AB2010204 | ||
| P8 | CTCGACTTCATTAACTTATTC | |||
| P9 | TATACATatgtcccgcattacaattg | NdeI | pET28b | L. amylovorus CGMCC 11056 |
| P10 | TATGCTCGAGctagaataatggtttt | XhoI | ||
| P11 | TGCGCATatgtctcgcattacaattg | NdeI | pET28b | L. acidophilus CCTCC AB2010208 |
| P12 | TATACTCGAGttaaaataggggcttc | XhoI | ||
| P13 | TGCGCATatgaaagtagaaattaaac | NdeI | pET28b | L. farciminis CCTCC AB2016237 |
| P14 | TATACTCGAGttaatccaacatgaat | XhoI | ||
| P15 | TGCGCATatggaagttgcaatcaag | NdeI | pET15b | L. fermentum CCTCC AB2010204 |
| P16 | TCGCGGATCCttagtttaagaaatcg | BamHI | ||
The restriction sites are underlined, and the nucleotides pairing with the potential FAE gene sequence are in lowercase letters.