Table 3.
Effect of various metal ions on relative activity of the FAEs.
| Metal ions | Relative activity (%) | |||||||
|---|---|---|---|---|---|---|---|---|
| FaeLam | FaeLac | FaeLfa | FaeLfe | |||||
| 1 mM | 10 mM | 1 mM | 10 mM | 1 mM | 10 mM | 1 mM | 10 mM | |
| K+ | 99.2 ± 4.3 | 84.4 ± 4.3 | 97.5 ± 1.9 | 78.5 ± 10.8 | 102.8 ± 2.4 | 91.9 ± 0.2 | 90.3 ± 1.4 | 74.9 ± 2.3 |
| Ca2+ | 99.9 ± 0.7 | 86.6 ± 8.7 | 99.5 ± 1.6 | 89.8 ± 1.9 | 101.9 ± 4.3 | 82.7 ± 10.2 | 90.3 ± 0.3 | 79.1 ± 0.2 |
| Fe2+ | 57.1 ± 5.2 | 0 | 64.8 ± 13.3 | 5.5 ± 0.6 | 18.6 ± 4.4 | 3.6 ± 1.1 | 24.3 ± 2.8 | 0 |
| Fe3+ | 75.6 ± 13.1 | 0 | 75.7 ± 0.4 | 0 | 40.1 ± 8.5 | 1.8 ± 0.2 | 62.5 ± 2.8 | 0 |
| Mg2+ | 103.9 ± 1.5 | 84.6 ± 2.0 | 93.6 ± 0.4 | 90.6 ± 0.7 | 100.1 ± 1.1 | 93.8 ± 1.0 | 104.6 ± 6.4 | 82.3 ± 0.7 |
| Cu2+ | 63.6 ± 7.1 | 0 | 23.1 ± 4.5 | 3.4 ± 0.5 | 5.3 ± 0.5 | 2.12 ± 0.1 | 0 | 0 |
| Zn2+ | 88.0 ± 5.6 | 67.5 ± 1.3 | 84.9 ± 2.3 | 18.7 ± 0.3 | 103.1 ± 0.9 | 11.2 ± 0.1 | 91.4 ± 4.1 | 0.9 ± 0.3 |
| Ba2+ | 101.7 ± 6.3 | 82.2 ± 1.8 | 96.2 ± 0.5 | 82.1 ± 1.2 | 101.4 ± 1.8 | 84.8 ± 0.1 | 104.2 ± 3.2 | 68.5 ± 5.1 |
| Mn2+ | 98.5 ± 10.2 | 66.2 ± 4.6 | 93.2 ± 3.3 | 77.7 ± 0.5 | 101.3 ± 2.4 | 82.6 ± 1.4 | 103.2 ± 4.6 | 70.5 ± 1.8 |
| Co2+ | 93.1 ± 0.5 | 74.0 ± 4.6 | 106.2 ± 5.9 | 85.2 ± 7.9 | 96.5 ± 0.4 | 85.9 ± 0.5 | 101.7 ± 7.8 | 88.8 ± 1.0 |
FAE activity in the absence of metal ions was regarded as 100%.