Figure 2.

Genomic Southern-blot analysis of transgenic plants. Genomic DNAs extracted from individual regenerated plants of independent events were digested by HindIII, and separated on 1% agarose gel. Different lanes under the same transgenic event (named as TG) represent individual plants of this event. A non-transgenic plant (NT lane) and BAC SB40L16 DNA (BAC lane) was included as negative and positive control, respectively. A 1 kb ladder was used as a size reference on both sides of the gel. Fractionated DNA was transferred onto a nylon membrane, and hybridized with ³²P-labeled probes derived from BAC vector pBeloBAC II (A) or kafirin gene-coding region (B). The arrows in (A) indicate two bands detected from event TG14 by BAC sequences; the arrows in (B) indicate the two kafirin-specific bands detected from event TG14.