Figure 4.

IEF gel analysis of alcohol-soluble proteins from event TG14. Seed proteins containing the zein/kafirin prolamin fraction were extracted with 70% ethanol/1% 2-mercaptoethanol. About 5 μg of 10 T2 (lanes 1–10) and 10 T3 (lanes 11–20) individual seed protein samples of transgenic event TG14 were loaded onto the gel and subjected to isoelectric focusing as described under Materials and Methods. Two sorghum seed samples (SB lanes) and three non-transgenic seed samples (NT lanes) were included as positive or negative controls for kafirins. An arrow on the right-hand side indicates the position of the 22 kDa kafirin band.