Figure 3.
The influence of TQ5 on collagen-induced cyclic nucleotides formation and Akt phosphorylation. (A) Washed platelets (3.6 × 108 cells/mL) were preincubated with 10 µM NTG, 0.1 nM PGE1, or 5 µM TQ5 in the absence or presence of 20 µM ODQ or 100 µM SQ22536, and 1 µg/mL collagen was subsequently added to trigger platelet aggregation; (B) Platelets (1.2 × 109 cells/mL) were pretreated with 3 and 5 µM TQ5 and 1 µg/mL collagen was subsequently added to induce platelet activation; The cells were collected, and subcellular extracts were analyzed for Akt phosphorylation. Data are presented as the means ± standard error of mean (S.E.M). (n = 4). ***p < 0.001 compared with the control group; # p < 0.05 compared with the positive control group (collagen only).